Flow cytometry is a powerful tool for the multiparameter analysis of cells of all types. One high powered high speed flow cytometer and as well as a four color analyzer are available in our flowcytometry core facility at CSCR.
The Flow Cytometry Shared facility provides cell analysis and cell sorting services for the investigators in the stem cell research at the Centre for Stem Cell Research as well as off site researchers. The goal of the Flow Cytometry Shared facility is to (1) promote stem cell research and training researchers in the cell based assays using flowcytometers (2) innovating technique and analysis for cell assays to use in the Centre for Stem Cell Research; and (3) support early clinical trial analysis. The Flow Cytometry core facility enhances the productivity and effectiveness of research by promoting high-speed cell sorting and acquisition, ensuring the highest data quality and consistency, carrying out efficient cell sorting, providing reliable data management, educating and training researchers and consulting in experiment design. Major applications of flow cytometry services include Immuno phenotyping, Cell cycle analysis, stem cells, rare events and other cell sorting.
The BD FACS Aria 1 is an analyzer and cell sorter with three lasers, an argon blue laser 488nm (7 colors), Red HeNe laser 633nm (2 colors) and violet laser 405nm (2 colors) and capable of detecting 9 fluorescent signals e.g. FITC, PE, PE-Texas Red or Quantum Dot 605, PerCP-Cy5.5, PE-Cy7, APC, APC-Cy7, Sp1-Blue, Sp2-Red. The FACS Aria can perform 2 and 4 way sorting. This machine can process 70,000 cells per second for cells >10 Ám via 70 micron and 100 Ám nozzle sizes at 70 and 20 psi respectively.
The BD FACSCalibur is an analyzer with two lasers, an argon blue laser 488nm (3 colors), Red HeNe laser 633nm (1 color) and capable of detecting 4 fluorescent signals e.g. FITC, PE, PerCP, APC. This machine has cell quest pro software to acquire and analyze the data.
A separate work station for offline data analysis using FlowJo is also available.
The imaging facility at the CSCR presently has state of the art DMI 6000 fully automated inverted microscope with a motorized focus and stage.
This, along with a motorized filter wheel for Hoechst/ DAPI, FITC and Texas Red and a high resolution DFC290 digital camera allows real time multi-label image acquisition in multi-dimensions. The microscope is coupled to the AF 6000 system with dual monitors for fast image acquisition and processing. Post acquisition processing can be carried out on the AF 6000 software suite, which includes the Deconvolution, 3D Visualization, Colocalization and Dye Finder modules.
The MZ16FA stereomicroscope is a fully motorized, automated, fully apochromatic fluorescence stereo microscope with a four-set filter wheel. It is equipped with a camera, and has computerized controls for the filter changer, zoom, focus, UV shutter, and double iris shutter, with a zoom of 16:1, and a magnification of 115X, and a resolution of 849Lp/mm.
It will be used for micro dissections with regular and fluorescent embryos among other applications. Individual labs are equipped with DMIL inverted microscopes. In addition, the M651 surgical operating microscope, trinocular research microscope, and two BME binocular microscopes are available.
Gene / DNA Sequencing facility
The DNA Sequencing Facility provides DNA sequencing services to investigators at Centre for Stem Cell Research, as well as for off site researchers. This is equipped with the ABI Prism 3130 Genetic Analyzer, a 4-capillary fluorescence-based DNA analysis system.
In addition to DNA sequencing, the 3130 system supports a variety of applications including comparative sequencing, DNA fragment analysis, and genotyping.
Skilled staff members are ready to lend their expertise in sequencing PCR products, plasmids, and BACs.
This instrument is capable of reading up to 800 base pairs with 95 percent accuracy.
Real-time - PCR Facility
The molecular core facility has Life tech 7500 and Life tech Quantstudio Real Time PCR from available for people to reserve and use.
Ultra & High speed centrifuge
High speed centrifuge: Beckman Coulter Avanti J-30 high-speed centrifuge with the following rotors: JLA - 10.50, JA-30.50 and JLA-9.10.
Ultra-centrifuge: Beckman Coulter Optima L-100K Ultra-centrifuge with the following rotors: NVT 90 rotor package, SW 32 Ti, 70 Ti.
The histopathology laboratory, located on the first floor of the CSCR, has been designed to process paraffin embedded and routine and special stains, immunocytochemistry, rapid tissue sections, immunohistochemistry, cytology and routine microscopy.
A grossing station is present. The equipment comprises:
- semi rotarized rotary microtome (Leica RM 2245),
- heated paraffin embedding system & cold plate (Leica EG 1150 H & C)
- automated tissue processor (Leica TP1020),
- cryostat (Leica CM 1900 UV),
- Shandon cytospin 4 cytocentrifuge
- fume exhaust hood.
We recently obtained the approval (AERB/RSD/Res-Pln/TN-01/2009)from Atomic Energy Regulatory Board to use radioactive materials in our centre, we have thus established a dedicated facility to handle alpha and beta radio isotopes. This facility is equipped with following detection and quantification equipments.
- Liquid scintillation counter (Perkin Elmer - TriCarb 2810TR) to detect the concentration and the half life of the radioisotopes.
- Phosphor Imager (GE - Storm 865) to read the radiation exposed screens.
- GM-counter (Ludlum) a handheld detector to detect the spill.